Anti-Mesothelin [11-25]

SKU: orb1089941

Description

Rabbit monoclonal antibody to Mesothelin

Research Area

Cancer Biology

Images & Validation

• Tested Applications: ELISA, FC, IP
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: 11-25
• Immunogen: The original antibody was generated by intraperitoneally immunizing BALB/c mice with the purified recombinant mesothelin protein.
• Target: Mesothelin
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

MSLN; CAK1 antigen; Pre-pro-megakaryocyte-potentiating factor

Flow cytometry using the Anti-Mesothelin antibody 11-25. Paraformaldehyde fixed HeLa cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 11-25 (orb1089941, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-Mesothelin 11-25. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of 11-25 (orb1089941) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089941, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

Mesothelin

UniProt

Q13421