MEF2C Antibody

SKU: orb256682

Description

The MEF2C Antibody is suitable for ChIP, IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MEF2C. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

• Tested Applications: ChIP, IF, IHC, WB
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Reactivity: Human, Mouse, Porcine, Zebrafish

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MEF2C. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Myocyte-specific enhancer factor 2C

Western blot analysis of MEF2C expression in zebrafish (A) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 55 kD)

Immunohistochemical analysis of MEF2C staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MEF2C staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.

Quick Database Links

UniProt

Q06413, Q8CFN5

NCBI Gene ID

4208, 17260

References

Mara Bouwman # 1, Dennis E M de Bakker # 1 2, Hessel Honkoop 1, Alexandra E Giovou 3, Danielle Versteeg 1, Arie R Boender 3 4, Phong D Nguyen 1 5, Merel Slotboom 1, Daniel Colquhoun 6, Marta Vigil-Garcia 1, Lieneke Kooijman 1, Rob Janssen 3, Ingeborg B Hooijkaas 3, Marie Günthel 3, Kimberly J Visser 1, Mischa Klerk 3, Lorena Zentilin 7, Mauro Giacca 7 8, Jan Kaslin 6, Gerard J J Boink 3 4 9, Eva van Rooij 1 10, Vincent M Christoffels 3, Jeroen Bakkers Cross-species comparison reveals that Hmga1 reduces H3K27me3 levels to promote cardiomyocyte proliferation and cardiac regeneration Nat Cardiovasc Res, (2024)

PubMed: 39747457

Applications

IHC

Reactivity

Human

Konstantinos Lekkos 1 2, Zhilian Hu 1 2, Phong D Nguyen 3 4, Hessel Honkoop 3, Esra Sengul 1 2, Rita Alonaizan 1 2 5, Jana Koth 6, Jun Ying 1 2 7, Madeleine E Lemieux 8, Alisha Kenward 1 2, Sean Keeley 9, Bastiaan Spanjaard 10 11, Brett W C Kennedy 2, Xin Sun 1 2, Katherine Banecki 2, Helen G Potts 2, Gennaro Ruggiero 1 2, James Montgomery 12, Daniela Panáková 10 13 14, Jan Philipp Junker 10 11 13, Lisa C Heather 2, Xiaonan Wang 15, Juan Manuel Gonzalez-Rosa 9 16, Jeroen Bakkers 3 17, Mathilda T M Mommersteeg 18 19 Oxidative phosphorylation is required for cardiomyocyte re-differentiation and long-term fish heart regeneration Nat Cardiovasc Res ., (2025)

PubMed: 41034455

Applications

IF

Reactivity

Zebrafish

Honkoop, Hessel et al. Live imaging of adult zebrafish cardiomyocyte proliferation ex vivo Development, (2021)

PubMed: 34397091

Applications

IF

Reactivity

Zebrafish

de Bakker, Dennis E. M. et al. Prrx1b restricts fibrosis and promotes Nrg1-dependent cardiomyocyte proliferation during zebrafish heart regeneration Development, (2021)

PubMed: 34486669

Applications

IF

Reactivity

Zebrafish