Anti-MEF2C Antibody

• Catalog Number: orb256682
• Category: Antibodies
• Description: Rabbit polyclonal antibody to MEF2C
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ChIP, IF, IH, WB
• Reactivity: Human, Mouse, Porcine, Zebrafish
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human MEF2C. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-200, IF/ICC: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: MEF2C
• Entrez: 17260, 4208
• UniProt ID: Q8CFN5, Q06413
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti Myocyte-specific enhancer factor 2C antibody
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

References

Mara Bouwman # 1, Dennis E M de Bakker # 1 2, Hessel Honkoop 1, Alexandra E Giovou 3, Danielle Versteeg 1, Arie R Boender 3 4, Phong D Nguyen 1 5, Merel Slotboom 1, Daniel Colquhoun 6, Marta Vigil-Garcia 1, Lieneke Kooijman 1, Rob Janssen 3, Ingeborg B Hooijkaas 3, Marie Günthel 3, Kimberly J Visser 1, Mischa Klerk 3, Lorena Zentilin 7, Mauro Giacca 7 8, Jan Kaslin 6, Gerard J J Boink 3 4 9, Eva van Rooij 1 10, Vincent M Christoffels 3, Jeroen Bakkers Cross-species comparison reveals that Hmga1 reduces H3K27me3 levels to promote cardiomyocyte proliferation and cardiac regeneration Nat Cardiovasc Res, (2024)

PubMed: 39747457

Applications

IHC

Reactivity

Human

de Bakker, Dennis E. M. et al. Prrx1b restricts fibrosis and promotes Nrg1-dependent cardiomyocyte proliferation during zebrafish heart regeneration Development, (2021)

PubMed: 34486669

Applications

IF

Reactivity

Zebrafish

Honkoop, Hessel et al. Live imaging of adult zebrafish cardiomyocyte proliferation ex vivo Development, (2021)

PubMed: 34397091

Applications

IF

Reactivity

Zebrafish

Western blot analysis of MEF2C expression in zebrafish (A) whole cell lysates. (Predicted band size: 51 kD; Observed band size: 55 kD)

Immunohistochemical analysis of MEF2C staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MEF2C staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.