MCM3 Rabbit Monoclonal Antibody

SKU: orb548060

Description

Anti-MCM3 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, Flow Cytometry applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Epigenetics & Chromatin, Molecular Biology

Images & Validation

• Tested Applications: FC, ICC, IF, IHC, WB
• Dilution Range: WB 1:500-2000 IHC 1:50-200 ICC/IF 1:50-200 FC 1:50
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AFCC-13
• Immunogen: A synthesized peptide derived from human MCM3 Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells.
• Target: DNA replication licensing factor MCM3
• Molecular Weight: 41 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Rabbit IgG in stabilizing components, phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: 0.5mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Proline-, glutamic acid- and leucine-rich protein 1; Modulator of non-genomic activity of estrogen receptor; Transcription factor HMX3; PELP1; HMX3, MNAR

Western blot analysis of MCM3 expression in (1) K562 cell lysate; (2) RAW264.7 cell lysate using anti-MCM3 antibody (orb548060). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MCM3 antigen affinity purified polyclonal antibody (Catalog # orb548060) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for MCM3.

Quick Database Links

Gene Symbol

DNA replication licensing factor MCM3

UniProt

P25205