MCM2 Antibody

• Catalog Number: orb1474241
• Category: Antibodies
• Description: The MCM2 Antibody is suitable for FC, IF, IHC, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant fragment of human MCM2 expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human, Mouse
• Form/Appearance: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Purified recombinant fragment of human MCM2 expressed in E. Coli.
• UniProt ID: P49736, P97310
• Tested applications: FC, IF, IHC, IP, WB
• Dilution range: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), FC (1/50 - 1/100)
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: BM28; CCNL1; CDCL1; KIAA0030; DNA replication lice
• Note: For research use only
• Entrez: 4171, 17216

Western blot analysis of MCM2 expression in Jurkat (A), K562 (B), NIH3T3 (C), Hela (D) whole cell lysates. (Predicted band size: 102 kD; Observed band size: 125 kD)

Immunohistochemical analysis of MCM2 staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.142). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of MCM2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.