MALT1 Monoclonal Antibody

• Catalog Number: orb547801
• Category: Antibodies
• Description: MALT1 Monoclonal Antibody
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human MALT1 Enhances BCL10-induced activation of NF-kappa-B. Involved in nuclear export of BCL10. Binds to TRAF6, inducing TRAF6 oligomerization and activation of its ligase activity. Has ubiquitin ligase activity. MALT1-dependent BCL10 cleavage plays an important role in T-cell antigen receptor-induced integrin adhesion.
• UniProt ID: Q9UDY8
• MW: 40-48 kDa
• Tested applications: FC, ICC, IF, WB
• Application notes: WB 1:500-2000ICC/IF 1:50-200FC 1:20. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: ADDA-13
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: E3 ubiquitin-protein ligase NEDD4-like; 6.3.2.-; N
• Note: For research use only

Western blot analysis of MALT1 expression in Jurkat cell lysate using anti-MALT1 antibody (orb547801). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MALT1 antigen affinity purified polyclonal antibody (Catalog # orb547801) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for MALT1.