Anti-MAGEA3 [21B4]

• Catalog Number: orb1463347
• Category: Antibodies
• Description: Human monoclonal antibody to MAGEA3
• Species/Host: Human
• Clonality: Monoclonal
• Clone Number: 21B4
• Tested applications: ELISA, FC, IHC, IP, WB
• Reactivity: Human
• Isotype: Human IgG
• Immunogen: The original antibody was isolated from a patient suffering from a tumor expressing a tumor specific antigen like MAGE-A3.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: MAGEA3
• UniProt ID: P43357, P43360
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: MAGE-3; MAGE3; MAGE 3; CT1.3; MAGE-3 antigen; Mela
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-MAGE-3 antibody 21B4. HeLa cells were stained with an isotype control (orb256390, black line) or the rabbit-chimeric version of 21B4 (orb1463347, blue line) at a concentration of 1 µg/ml for 30 mins at RT. After washing, bound antibody was detected using Alexa Fluor 488® conjugated goat anti-rabbit IgG and cells analysed on a FACSCanto flow-cytometer.

Immunofluorescence staining of HeLa cells with anti-MAGE-3 21B4. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells stained with the chimeric rabbit IgG version of 21B4 (orb1463347) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1463347, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-MAGE-3 antibody 21B4. HeLa(A) (0.01 µg/ml) cells lysate and HeLa(B) (0.01 µg/ml) cells nuclear lysate (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of 21B4 (orb1463347) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.