Anti-Ly6G/Ly6C [RB6-8C5]

• Catalog Number: orb758800
• Category: Antibodies
• Description: Rat monoclonal antibody to Ly6G
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: RB6-8C5
• Tested applications: FC, IHC, WB
• Reactivity: Mouse
• Isotype: IgG2a
• Immunogen: This antibody was raised in Lou-M rats using a popliteal lymph node immunisation and fusion protocol as described by Martiniello et al (1994)
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Ly6G/Ly6C
• UniProt ID: P35461
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Gr-1; Gr1; Ly-6G; ly-6G.1; ly6C
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-Ly6G/Ly6C antibody RB6-8C5. Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of RB6-8C5 (orb758799, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes using anti-Ly6G/Ly6C antibody RB6-8C5. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of RB6-8C5 (orb758799) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining in subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758799, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.