LRP130 Rabbit Polyclonal Antibody

SKU: orb412181

Description

The LRP130 Antibody is suitable for IF, IHC, IP, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human LRP130 .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Cell Biology

Images & Validation

• Tested Applications: IF, IHC, IP, WB
• Dilution Range: WB: 1:500-2000, IHC-P: 1:50-200, IP: 1:50-100
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human LRP130
• Target: LRPPRC
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

LRP130; Leucine-rich PPR motif-containing protein mitochondrial; 130 kDa leucine-rich protein; LRP 130; GP130

Western blot analysis of LRP130 expression in Hela (A), HepG2 (B), mouse heart (C), mouse liver (D) whole cell lysates. (Predicted band size: 157 kD; Observed band size: 130 kD)

Immunofluorescent analysis of LRP130 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

LRPPRC

UniProt

Q6PB66, Q5SGE0, P42704

NCBI Gene ID

10128, 72416, 313867