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Description
Images & Validation
−| Tested Applications | IP, WB |
|---|---|
| Dilution range | WB: 1-500-1-1000, IP: 1-10-1-100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human LRP11. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−LRP11 Rabbit Polyclonal Antibody [orb185007]
WB
Human
Rabbit
Polyclonal
Unconjugated
50 μl, 200 μl, 100 μlLRP11 Rabbit Polyclonal Antibody [orb580987]
WB
Bovine, Canine, Equine, Guinea pig, Mouse, Porcine, Rabbit, Rat
Human
Rabbit
Polyclonal
Unconjugated
100 μl

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Western blot analysis of LRP11 expression in A431 (A), HepG2 (B), Jurkat (C) whole cell lysates. (Predicted band size: 53 kD; Observed band size: 49 kD)

Immunoprecipitation of LRP11 from 0.5 mg Jurkat whole cell extract lysate, using 5 ug of Anti-LRP11 Antibody and 50 ul of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10 min, Jurkat whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10 min under agitation. Proteins were eluted by addition of 40 ul SDS loading buffer and incubated for 10 min at 70°C; 10 ul of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with Anti-LRP11 Antibody.
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Protocol Information
LRP11 Antibody (orb215330)
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