Lin28 LIN28A Rabbit Monoclonal Antibody

SKU: orb547916

Description

Anti-Lin28 LIN28A Rabbit Monoclonal Antibody. Tested in WB, IP, Flow Cytometry applications. This antibody reacts with Human.

Images & Validation

• Tested Applications: FC, IP, WB
• Reactivity: Human
• Application Notes:
  WB 1:500-1:2000IP 1:50FC 1:50. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone No.: AAAG-12
• Immunogen: A synthesized peptide derived from human Lin28
• Molecular Weight: 26 kDa
• Purification: Affinity-chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Disclaimer: For research use only

Alternative Names

Protein lin-28 homolog A; Lin-28A; Zinc finger CCHC domain-containing protein 1; LIN28A; CSDD1, LIN28, ZCCHC1

Western blot analysis of Lin28 expression in NCCIT cell lysate (orb547916). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LIN28A monoclonal antibody (Catalog # orb547916) overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for LIN28A.

Quick Database Links

UniProt

Q9H9Z2