LFA3/CD58 Rabbit Polyclonal Antibody

SKU: orb371642

Description

LFA3/CD58 Rabbit Polyclonal Antibody

Research Area

Disease Biomarkers, Immunology & Inflammation

Images & Validation

• Tested Applications: FC, IHC, WB
• Dilution Range: Western blot, 0.1-0.5μg/ml Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml Flow Cytometry, 1-3μg/1x10^6cells
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Immunogen: E. coli-derived human LFA3 recombinant protein (Position: F29-R215).
• Target: Lymphocyte function-associated antigen 3
• Molecular Weight: 26 kDa
• Purification: Immunogen affinity purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Lymphocyte function-associated antigen 3; Ag3; Surface glycoprotein LFA-3; CD58; CD58; LFA3

Western blot analysis of LFA3 expression in HELA whole cell lysates (lane 1) and K562 whole cell lysates (lane 2). LFA3 at 35 KD was detected using rabbit anti-LFA3 Antigen Affinity purified polyclonal antibody at 0.5 µg/mL. The blot was developed using chemiluminescence (ECL) method.

Flow Cytometry analysis of Hela cells using anti-LFA3 antibody. Overlay histogram showing Hela cells (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-LFA3 Antibody (1 µg/1x106 cells) for 30 min at 20°C. DyLight488 conjugated goat anti-rabbit IgG (5-10 µg/1x106 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1 µg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.

LFA3 was detected in paraffin-embedded sections of human tonsil tissues using rabbit anti-LFA3 Antigen Affinity purified polyclonal antibody at 1 µg/mL. The immunohistochemical section was developed using SABC method.

Quick Database Links

Gene Symbol

Lymphocyte function-associated antigen 3

UniProt

P19256