Anti-Lewis a,b [HEA164]

• Catalog Number: orb758886
• Category: Antibodies
• Description: Mouse monoclonal antibody to Lewis a,b
• Target: Lewis a,b
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: IgM
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was raised by immunising mice with human carcinoma cells.
• Tested applications: FC, IF, IHC, IP, WB
• Clone Number: HEA164
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Blood Group Lewis a/b; lewis a; lewis b
• Note: For research use only

Immunofluorescence staining of fixed Caco-2 cells with anti-Lewis a, b antibody HEA164. Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of HEA164 (orb758887) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758887, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-Lewis a, b antibody HEA164 Human colorectal carcinoma tissue lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HEA164 (orb758887) at 0.001 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.