Anti-L3MBTL1 [RAB-C213]

• Catalog Number: orb1089952
• Category: Antibodies
• Description: Rabbit polyclonal antibody to L3MBTL1
• Target: L3MBTL1
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: Human IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was obtained by recombinant antibody (rAb) phage display recognizing L3MBTL1 protein under non-denaturing conditions.
• UniProt ID: Q9Y468
• Tested applications: ChIP, ELISA, FC, IF, IP
• Clone Number: RAB-C213
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: L3MBTL1; Lethal 3 malignant brain tumor-like prote
• Research Area: Cancer Biology, Cell Biology, Immunology & Inflamm
• Note: For research use only

Flow cytometry using the Anti-L3MBTL1 antibody RAB-C213. Paraformaldehyde fixed Kelly cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of RAB-C213 (orb1089952, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Kelly cells with anti-L3MBTL1 RAB-C213. Immunofluorescence analysis of paraformaldehyde fixed Kelly cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C213 (orb1089952) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089952, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.