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Anti-L3MBTL1 [RAB-C213]

SKU: orb1089952

Description

Rabbit polyclonal antibody to L3MBTL1

Research Area

Cancer Biology, Cell Biology, Immunology & Inflammation

Images & Validation

Tested ApplicationsChIP, ELISA, FC, IF, IP
ReactivityHuman

Key Properties

HostHuman
ClonalityMonoclonal
IsotypeHuman IgG
Clone No.RAB-C213
ImmunogenThis antibody was obtained by recombinant antibody (rAb) phage display recognizing L3MBTL1 protein under non-denaturing conditions.
TargetL3MBTL1
PurityPurified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

L3MBTL1; Lethal 3 malignant brain tumor-like protein 1; Lethal(3)malignant brain tumor-like protein 1; H-l(3)mbt; H-l(3)mbt protein; L(3)mbt-like; L(3)mbt protein homolog; anti-L3MBTL1-RAB-C213; L3MBTL1-A003-1453

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Anti-L3MBTL1 [RAB-C213]

Flow cytometry using the Anti-L3MBTL1 antibody RAB-C213. Paraformaldehyde fixed Kelly cells permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of RAB-C213 (orb1089952, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Anti-L3MBTL1 [RAB-C213]

Immunofluorescence staining of Kelly cells with anti-L3MBTL1 RAB-C213. Immunofluorescence analysis of paraformaldehyde fixed Kelly cells permeabilized with 0.15% Triton stained with the chimeric rabbit IgG version of RAB-C213 (orb1089952) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089952, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

UniProt Details

No UniProt data available

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Protocol Information

FC
Flow Cytometry
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IF
Immunofluorescence
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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IP
Immunoprecipitation
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ChIP
Chromatin Immunoprecipitation
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Anti-L3MBTL1 [RAB-C213] (orb1089952)

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Available Sizes

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0.2 mg
$ 600.00
DispatchUsually dispatched within 5-10 working days
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