Anti-Klotho Antibody

• Catalog Number: orb667095
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Klotho
• Target: KL
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant fusion protein of human Klotho. The exact sequence is proprietary.
• UniProt ID: O35082, Q9UEF7, Q9Z2Y9
• Tested applications: IF, IH, WB
• Dilution range: WB: 1-500-2000, IHC-P: 1-50-200, IF: 1-50-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-Klotho antibody
• Note: For research use only
• Entrez: 16591, 83504, 9365

Western blot analysis of Klotho expression in DU145 (A), mouse brain (B), rat kidney (C) whole cell lysates. (Predicted band size: 116 kD; Observed band size: 116; 62 kD)

Immunohistochemical analysis of Klotho staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Klotho staining in human placenta tissues. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a Alexa Fluor 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.