KLF4 Mouse Monoclonal Antibody

SKU: orb1474158

Description

The KLF4 Antibody is suitable for FC, IF, WB. It is a Monoclonal, Unconjugated antibody which raised against Recombinant fusion protein of human KLF4. The exact sequence is proprietary. Purification: This antibody is purified through a protein G column.

Images & Validation

• Tested Applications: FC, IF, WB
• Dilution Range: WB (1/1000 - 1/2000)
• Reactivity: Human

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b kappa
• Immunogen: Recombinant fusion protein of human KLF4. The exact sequence is proprietary.
• Target: KLF4
• Purification: This antibody is purified through a protein G column.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EZF; GKLF; Krueppel-like factor 4; Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor

Western blot analysis of KLF4 expression in recombincant protein (A) whole cell lysates. (Predicted band size: 54 kD; Observed band size: 62 kD)

Immunofluorescent analysis of KLF4 staining in Hela cells transfected with pMX constructs. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Flow cytometric analysis of Hela cells using Anti-KLF4 Antibody. The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were incubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody at 37 °C for 60 min. The secondary antibody Goat Anti-Mouse IgG (H&L) - AF594 was incubated at 37 °C for 40 min. Isotype control antibody (blue line) was used under the same condition.

Quick Database Links

Gene Symbol

KLF4

UniProt

O43474

NCBI Gene ID

9314