KIFC1 Rabbit Monoclonal Antibody

• Catalog Number: orb548362
• Category: Antibodies
• Description: KIFC1 Rabbit Monoclonal Antibody
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 0.5mg/ml
• Purification: Affinity-chromatography
• Immunogen: A synthesized peptide derived from human KIFC1
• UniProt ID: Q9BW19
• MW: 27 kDa
• Tested applications: ICC, IF, IHC, IP, WB
• Application notes: WB 1:500-2000IHC 1:50-200ICC/IF 1:50-200IP 1:20. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: EI-11
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Kinesin-like protein KIFC1; Kinesin-like protein 2
• Note: For research use only

Immunohistochemical analysis of paraffin-embedded human thyroid carcinoma, using KIFC1 Antibody (orb548362) KIFC1 was detected in paraffin-embedded tissue section. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-KIFC1 Antibody (orb548362) overnight at 4 Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # orb90444) with DAB as the chromogen.

Western blot analysis of KIFC1 expression in (1) HeLa cell lysate; (2) HepG2 cell lysate (orb548362). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-KIFC1 monoclonal antibody (Catalog # orb548362) overnight at 4 then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90503) with Tanon 5200 system. A specific band was detected for KIFC1.