KIF5A Rabbit Polyclonal Antibody

SKU: orb412173

Description

The KIF5A Antibody is suitable for IF, WB. It is a Polyclonal, Unconjugated antibody which raised against Recombinant fusion protein of human KIF5A .Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Neuroscience

Images & Validation

• Tested Applications: IF, WB
• Dilution Range: WB: 1:500-2000
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Recombinant fusion protein of human KIF5A
• Target: KIF5A
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NKHC1; Kinesin heavy chain isoform 5A; Kinesin heavy chain neuron-specific 1; Neuronal kinesin heavy chain; NKHC

Western blot analysis of KIF5A expression in mouse brain (A), rat brain (B) whole cell lysates. (Predicted band size: 117 kD; Observed band size: 129 kD)

Immunofluorescent analysis of KIF5A staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

Gene Symbol

KIF5A

UniProt

Q12840, P33175, Q6QLM7

NCBI Gene ID

3798, 314906, 16572