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Anti-Kappa Opioid Receptor (Phospho-S369) Antibody

Catalog Number: orb393127

DispatchUsually dispatched within 5-10 working days
$ 160.00
Catalog Numberorb393127
CategoryAntibodies
DescriptionRabbit polyclonal antibody to OPRK1 (Phospho-S369)
Species/HostRabbit
ClonalityPolyclonal
Tested applicationsIF, IH, WB
ReactivityHuman, Mouse, Rat
ImmunogenKLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S369 of human Kappa Opioid Receptor protein. The exact sequence is proprietary.
Antibody TypePrimary Antibody
Dilution rangeWB: 1:500:1000
Form/AppearanceLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
ConjugationUnconjugated
TargetOPRK1
Entrez29335, 4986, 18387
UniProt IDP34975, P41145, P33534
SourceRabbit
StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesLiquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Alternative namesOPRK1; OPRK; Kappa-type opioid receptor; K-OR-1; K
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NoteFor research use only
Expiration Date12 months from date of receipt.
Anti-Kappa Opioid Receptor (Phospho-S369) Antibody

Western blot analysis of Kappa Opioid Receptor (Phospho-S369) expression in PC12 (A), mouse kidney (B), mouse muscle (C), rat kidney (D), rat muscle (E) whole cell lysates. (Predicted band size: 42 kD; Observed band size: 43 kD)

Anti-Kappa Opioid Receptor (Phospho-S369) Antibody

Immunohistochemical analysis of Kappa Opioid Receptor (Phospho-S369) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Anti-Kappa Opioid Receptor (Phospho-S369) Antibody

Immunofluorescent analysis of Kappa Opioid Receptor (Phospho-S369) staining in LO2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. Phalloidin - AF594 was used to stain Actin filaments (red). DAPI was used to stain the cell nuclei (blue).