Anti-JNK1/2/3 (Phospho-Y185) Antibody

• Catalog Number: orb665514
• Category: Antibodies
• Description: Rabbit polyclonal antibody to JNK1/2/3 (Phospho-Y185)
• Target: MAPK8; MAPK9; MAPK10
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y185 of human JNK1/2/3 protein. The exact sequence is proprietary.
• UniProt ID: P49186, P49187, Q91Y86, P45984, P49185, Q9WTU6, Q61831, P45983, P53779
• Tested applications: IH, WB
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Anti-MAPK8 antibody, anti-JNK1 antibody, anti-PRKM
• Note: For research use only
• Entrez: 116554, 5602, 5599, 25272, 5601, 50658, 26419, 26420
• Expiration Date: 12 months from date of receipt.

Western blot analysis of JNK1/2/3 (Phospho-Y185) expression in HCT116 (A), U87MG (B) whole cell lysates. (Predicted band size: 48; 52 kD; Observed band size: 54 kD)

Immunohistochemical analysis of JNK1/2/3 (Phospho-Y185) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.