Anti-JAK3 (Phospho-Y785) Antibody

• Catalog Number: orb665657
• Category: Antibodies
• Description: Rabbit polyclonal antibody to JAK3 (Phospho-Y785)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Primate
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y785 of human JAK3 protein. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1-500-1000, IHC-P: 1-50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: JAK3
• Entrez: 16453, 3718
• UniProt ID: P52333, Q62137
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: Anti-Tyrosine-protein kinase JAK3 antibody, anti-J
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of JAK3 (Phospho-Y785) expression in Myla2059 (A), K562 (B), Jurkat (C) whole cell lysates. (Predicted band size: 125 kD; Observed band size: 125 kD)

Immunohistochemical analysis of JAK3 (Phospho-Y785) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.