Anti-JAK2 Monoclonal Antibody

• Catalog Number: orb546990
• Category: Antibodies
• Description: Anti-JAK2 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat.
• Clonality: Monoclonal
• Species/Host: Rabbit
• Isotype: Rabbit IgG
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid
• Concentration: Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure.
• Immunogen: A synthesized peptide derived from human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2.
• UniProt ID: O60674
• MW: 131 kDa
• Tested applications: ICC, IF, IHC, IP, WB
• Application notes: WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50-100. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL
• Antibody Type: Primary Antibody
• Clone Number: COG-10
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Signal transducer and activator of transcription 3
• Note: For research use only

ICC staining JAK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

WB analysis of JAK2 using anti-JAK2 antibody in Jurkat Cell.

WB analysis of JAK2 using anti-JAK2 antibody in (1) MCF-7 Cell; (2) A549 Cell; (3) rat lung Tissue.

Immunofluorescent analysis of Jurkat cells, using JAK2 Antibody.