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Anti-JAK2 Monoclonal Antibody
Catalog Number: orb546990
| Catalog Number | orb546990 |
|---|---|
| Category | Antibodies |
| Description | Anti-JAK2 Monoclonal Antibody. Tested in WB, IHC, ICC/IF, IP applications. This antibody reacts with Human, Mouse, Rat. |
| Species/Host | Rabbit |
| Clonality | Monoclonal |
| Clone Number | COG-10 |
| Tested applications | ICC, IF, IHC, IP, WB |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Immunogen | A synthesized peptide derived from human JAK2 Phosphorylated STATs then form homodimer or heterodimers and translocate to the nucleus to activate gene transcription. For example, cell stimulation with erythropoietin (EPO) during erythropoiesis leads to JAK2 autophosphorylation, activation, and its association with erythropoietin receptor (EPOR) that becomes phosphorylated in its cytoplasmic domain. Then, STAT5 (STAT5A or STAT5B) is recruited, phosphorylated and activated by JAK2. |
| Antibody Type | Primary Antibody |
| Concentration | Actual concentration vary by lot. Use suggested dilution ratio to decide dilution procedure. |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 131 kDa |
| UniProt ID | O60674 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Alternative names | Signal transducer and activator of transcription 3 Read more... |
| Note | For research use only |
| Application notes | WB 1:500-1:2000IHC 1:50-1:200ICC/IF 1:50-1:200IP 1:50-100. Restore with deionized water (or equivalent) for reconstitution volume of 1.0 mL |
| Expiration Date | 12 months from date of receipt. |
ICC staining JAK2 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
WB analysis of JAK2 using anti-JAK2 antibody in Jurkat Cell.
WB analysis of JAK2 using anti-JAK2 antibody in (1) MCF-7 Cell; (2) A549 Cell; (3) rat lung Tissue.
Immunofluorescent analysis of Jurkat cells, using JAK2 Antibody.
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