Anti-IRE1 Antibody

• Catalog Number: orb515588
• Category: Antibodies
• Description: Mouse monoclonal antibody to ERN1
• Target: ERN1
• Clonality: Monoclonal
• Species/Host: Mouse
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: Purified recombinant fragment of human IRE1 expressed in E. Coli.
• UniProt ID: O75460
• Tested applications: IH, WB
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-500
• Antibody Type: Primary Antibody
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IRE1; Serine/threonine-protein kinase/endoribonucl
• Note: For research use only
• Entrez: 2081

Western blot analysis of IRE1 expression in Raji (A), Jurkat (B), Hela(C), HEK293T (D) whole cell lysates. (Predicted band size: 109 kD; Observed band size: 109 kD)

Immunohistochemical analysis of IRE1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.