Anti-IRE1 Antibody

• Catalog Number: orb515588
• Category: Antibodies
• Description: Mouse monoclonal antibody to ERN1
• Species/Host: Mouse
• Clonality: Monoclonal
• Tested applications: IH, WB
• Reactivity: Human
• Immunogen: Purified recombinant fragment of human IRE1 expressed in E. Coli.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500-1000, IHC-P: 1:100-500
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: ERN1
• Entrez: 2081
• UniProt ID: O75460
• Source: Mouse
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: IRE1; Serine/threonine-protein kinase/endoribonucl
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IRE1 expression in Raji (A), Jurkat (B), Hela(C), HEK293T (D) whole cell lysates. (Predicted band size: 109 kD; Observed band size: 109 kD)

Immunohistochemical analysis of IRE1 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.