Anti-Interleukin-4 (IL-4)

SKU: orb1125585

Description

Rabbit polyclonal antibody to Interleukin-4 (IL4)

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: To detect hIL-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection. IHC-P: Use at a concentration of 0.25 µg/ml. Neut: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/ml), a concentration of 0.03 - 0.05 µg/ml of this antibody is required. WB:To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Detects a band of approximately 15 kDa.
• Reactivity: Human
• Application Notes:
  ELISA: To detect hIL-4 by direct ELISA (using 100µl/well antibody solution) a concentration of at least 0.5µg/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection. IHC-P: Use at a concentration of 0.25 µg/ml. Neut: To yield one-half maximal inhibition [ND50] of the biological activity of hIL-4 (1.50 ng/ml), a concentration of 0.03 - 0.05 µg/ml of this antibody is required. WB:To detect hIL-4 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 µg/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant hIL-4 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Detects a band of approximately 15 kDa.

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: highly pure (>98%) recombinant hIL-4
• Purity: Affinity purified
• Purification: Affinity purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS buffer, pH 7.4. Reconstitute in sterile water at a concentration of >0.2 mg/ml.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only