Insulin Receptor (Phospho-Y1361) Antibody

SKU: orb393084

Description

The Insulin Receptor (Phospho-Y1361) Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1361 of human Insulin Receptor protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution range: WB: 1:500:1000, IHC-P: 1:100:200, IF/ICC: 1:100:500
• Reactivity: Human, Monkey, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y1361 of human Insulin Receptor protein. The exact sequence is proprietary.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Disclaimer: For research use only

Alternative Names

Insulin receptor; IR; CD220

Western blot analysis of Insulin Receptor (Phospho-Y1361) expression in THP1 (A), HEK293T (B) whole cell lysates. (Predicted band size: 156 kD; Observed band size: 95 kD)

Immunohistochemical analysis of Insulin Receptor (Phospho-Y1361) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunofluorescent analysis of Insulin Receptor (Phospho-Y1361) staining in HEK293T cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Quick Database Links

UniProt

P06213, P15208, P15127

NCBI Gene ID

16337, 3643

References

Lynda Bourebaba 1, Mikołaj Panek 2, Lilibeth Carolina Cárdenas Piedra 2, Nabila Bourebaba 2 Abnormal Fetuin-A levels in obese horses are associated with activated TLR4/NF-ƙB/MAPK axis and depleted FBXW7 E3 ubiquitin ligase Vet Res Commun, (2025)

PubMed: 41094197

Applications

WB

Reactivity

Equine