Anti-Insulin Receptor alpha [83-14]

• Catalog Number: orb613776
• Category: Antibodies
• Description: Rabbit monoclonal antibody to Insulin R alpha
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 83-14
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Bovine, Human, Porcine, Sheep
• Isotype: Mouse IgG
• Immunogen: This antibody was raised by immunizing mice with IM-9 lymphocytes followed by purified insulin receptor.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: Insulin Receptor alpha
• UniProt ID: P06213
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD220; Insulin Receptor alpha; HHF5; IR; HIR
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the anti-Insulin Receptor alpha antibody 83-14 Jurkat cells were stained with unimmunized rabbit IgG antibody (black line) or the rabbit-chimeric version of 83-14 (orb613776, blue line) at a concentration of 10 µg/ml for 30 mins at RT. After washing, bound antibody was detected using anti-rabbit IgG JK (FITC-conjugate) antibody (129936) at 2 µg/ml and cells analyzed on a FACSCanto flow-cytometer.