Anti-IL2R beta [5H4]

• Catalog Number: orb758864
• Category: Antibodies
• Description: Rabbit monoclonal antibody to IL2R beta
• Target: IL2R beta
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: Rat IgG
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was raised by immunising Lewis rats with transfected rat myeloma cell line YB2/0, expressing truncated murine IL-2R beta.
• UniProt ID: P16297
• Tested applications: FC
• Clone Number: 5H4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD122; IL-2Rβ; IL-2 receptor subunit beta; Interle
• Note: For research use only

Flow cytometry using the Anti-IL2R beta antibody 5H4. Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 5H4 (orb758864, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-IL2R beta antibody 5H4. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 5H4 (orb758864) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758864, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.