IL1B Biosimilar Antibody

• Catalog Number: orb1291211
• Category: Antibodies
• Description: Anti-IL1B(canakinumab biosimilar) mAb
• Target: IL1B
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Powder
• Purification: Purified from cell culture supernatant by affinity chromatography
• UniProt ID: P01584
• Tested applications: ELISA, FC
• Dilution range: ELISA 1:5000-10000 Flow Cyt 1:100
• Application notes: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution.
• Antibody Type: Biosimilar Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL-1 betaIL1F2
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human IL1B Protein, hFc Tag (orb1173751) can bind Anti-IL1B Neutralizing antibody (orb1291211) in a linear range of 0.61–156.25 ng/ml. In order to specifically detect orb1291211, mouse anti-human Fab-specific antibody was used as detection antibody.

Flow cytometry analysis under cell membrane permeable condition with 1 μg/mL Anti-IL1B (canakinumab biosimilar) mAb (orb1291211) on Expi293 cells transfected with Human IL1B protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).