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Catalog Number | orb1291118 |
---|---|
Category | Antibodies |
Description | Anti-IL17A(secukinumab biosimilar) mAb |
Target | IL17A |
Clonality | Monoclonal |
Species/Host | Human |
Isotype | IgG1 |
Conjugation | Unconjugated |
Reactivity | Human |
Form/Appearance | Powder |
Purification | Purified from cell culture supernatant by affinity chromatography |
UniProt ID | Q16552 |
Tested applications | ELISA, FC |
Dilution range | ELISA 1:5000-10000, Flow Cyt 1:100 |
Application notes | Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8% trehalose is added as protectants before lyophilization. Please see Certificate of Analysis for specific instructions of reconstitution. |
Antibody Type | Biosimilar Antibody |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Alternative names | CTLA-8CTLA8IL-17IL-17AIL17 |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Flow cytometry analysis under cell membrane permeable condition with 1 μg/mL Anti-IL17A (secukinumab biosimilar) mAb (orb1291118) on Expi293 cells transfected with Human IL17A protein (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram).
ELISA plate pre-coated by 2 μg/mL (100 μL/well) Human IL17A Protein, hFc Tag (orb1291066) can bind Anti-IL17A (secukinumab biosimilar) mAb (orb1291118) in a linear range of 6.40–160 ng/ml. In order to specifically detect orb1291118, mouse anti-human Fab-specific antibody was used as detection antibody.