Anti-IL-9 [RM9A4]

• Catalog Number: orb758848
• Category: Antibodies
• Description: Rat monoclonal antibody to IL-9
• Target: IL-9
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: IgG1
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was raised by immunising rats with recombinant full-length murine IL-9.
• UniProt ID: P15247
• Tested applications: ELISA, FC
• Clone Number: RM9A4
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Interleukin-9; IL9; Cytokine P40; T-cell growth fa
• Research Area: Immunology & Inflammation
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-9 antibody RM9A4. Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of RM9A4 (orb758847, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with orb758847. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.