Anti-IL-9 [MH9A4]

• Catalog Number: orb758846
• Category: Antibodies
• Description: Mouse monoclonal antibody to IL-9
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MH9A4
• Tested applications: ELISA, FC
• Reactivity: Human
• Isotype: IgG2b
• Immunogen: This antibody was raised by immunising C57Bl/6 mice with human IL-9 coupled to ovalbumin.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IL-9
• UniProt ID: P15248
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Interleukin-9; IL9; Cytokine P40; T-cell growth fa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-9 antibody MH9A4. Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MH9A4 (orb758845, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.