Anti-IL-9 [D9302C12]

• Catalog Number: orb758843
• Category: Antibodies
• Description: Hamster monoclonal antibody to IL-9
• Species/Host: Hamster
• Clonality: Monoclonal
• Clone Number: D9302C12
• Tested applications: Blocking, FC
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: This antibody was raised by immunising Armenian hamsters with Baculovirus-expressed, recombinant murine IL-9.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IL-9
• UniProt ID: P15247
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Interleukin-9; IL9; Cytokine P40; T-cell growth fa
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-9 antibody D9302C12. Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of D9302C12 (orb758844, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.