Anti-IL-8RA [SE2]

• Catalog Number: orb758960
• Category: Antibodies
• Description: Rabbit monoclonal antibody to IL-8RA
• Target: IL-8RA
• Clonality: Monoclonal
• Species/Host: Mouse
• Isotype: Mouse IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was raised by immunizing BALB/c mice with a BSA-conjugated peptide comprising of the N-terminal part of IL-8RA (amino acids 1-30).
• UniProt ID: P25024
• Tested applications: FC, IHC, WB
• Clone Number: SE2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CXCR-1; C-X-C chemokine receptor type 1; CXC-R1; C
• Note: For research use only

Flow cytometry using the Anti-IL-8RA antibody SE2. Paraformaldehyde fixed human peripheral blood monocytes were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of SE2 (orb758960, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed human peripheral blood leukocytes with anti-IL-8RA antibody SE2. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood leukocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of SE2 (orb758960) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758960, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-IL-8RA antibody SE2 Human lymph node tissue lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of SE2 (orb758960) at 0.1 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.