Anti-IL-6R [D7715A7]

• Catalog Number: orb758842
• Category: Antibodies
• Description: Rat monoclonal antibody to IL-6R
• Target: IL-6R
• Clonality: Monoclonal
• Species/Host: Rat
• Isotype: IgG2b
• Conjugation: Unconjugated
• Reactivity: Mouse
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Purity: Purified
• Immunogen: This antibody was raised by immunising Lewis rats with OKT4 cells.
• UniProt ID: P22272
• Tested applications: Blocking, FC
• Clone Number: D7715A7
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CD126; 15A7; IL-6Ra; IL6R; Interleukin-6 receptor
• Research Area: Cancer Biology, Immunology & Inflammation
• Note: For research use only

Flow-cytometry using the Anti-IL-6R antibody D7715A7. Mouse splenocytes were stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, shaded line) or the rabbit IgG version of D7715A7 (orb758841, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes using anti-IL-6R antibody D7715A7. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes immobilized on Shi-fix™ cover-slips and stained with the chimeric rabbit IgG version of D7715A7 (orb758841) at 10 µg/ml followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758841, DAPI, merged channels and an isotype control. The isotype control was stained with anti-Fluorescein antibody (orb256390) followed by Alexa Fluor® 488 secondary antibody.