IL-6 Antibody

• Catalog Number: orb315792
• Category: Antibodies
• Description: The IL-6 Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IL-6. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Monkey, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IL-6. The exact sequence is proprietary.
• UniProt ID: P08505, P05231, P20607
• Tested applications: IHC, WB
• Dilution range: WB: 1-500:1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IFNB2; Interleukin-6; IL-6; B-cell stimulatory fac
• Research Area: Epigenetics, Immunology
• Note: For research use only
• Entrez: 16193, 24498, 3569

Western blot analysis of IL-6 expression in Hela (A), U87MG (B), HuT78 (C), A549 (D), MCF7 (E) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 28; 18 kD)

Immunohistochemical analysis of IL-6 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Western blot analysis of IL-6 expression in U87MG (A), mouse brain (B), mouse kidney (C), rat kidney (D) whole cell lysates.