Anti-IL-5 [2E3]

• Catalog Number: orb669749
• Category: Antibodies
• Description: Mouse monoclonal antibody to IL-5
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 2E3
• Tested applications: ELISA
• Reactivity: Human
• Isotype: IgG2a
• Immunogen: This antibody was raised by immunising CAF1 mice with human recombinant IL-5.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IL-5
• UniProt ID: C9JQP9
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: interleukin 5; IL5; IL 5; Eosinophil differentiati
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-5 antibody 2E3. Human peripheral blood leukocytes were rested for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 2E3 (orb669750, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.