Anti-IL-18 Antibody

• Catalog Number: orb378127
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IL18.
• Target: IL18
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Human, Mouse, Rat
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Immunogen: Recombinant full length protein of human IL-18
• UniProt ID: P97636, Q14116, P70380
• Tested applications: IF, IP, WB
• Dilution range: WB: 1:500-2000
• Antibody Type: Primary Antibody
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IGIF; IL1F4; Interleukin-18; IL-18; Iboctadekin; I
• Note: For research use only
• Entrez: 3606, 16173, 29197

References

Alireza Barati 1 2, Sadegh Moghimi 3, Kiana Taghavi Zanjani 1, Mojde Rohani 4, Mehri Sohrabi Hesar 1, Arian Arfaie 1, Mohadese Ghezelche Khamsiyan 1, Javad Mahmoudi 2, Saeed Sadigh-Eteghad Acute Administration of Edaravone Improves Cognitive Impairment in a Mouse Model of mPFC Ischemia: Crosstalk Between Necroptosis, Neuroinflammation, and Antioxidant Defense Mol Neurobiol ., (2024)

PubMed: 39448519

Applications

IF

Reactivity

Mouse

Western blot analysis of IL-18 expression in Hela (A) whole cell lysates. (Predicted band size: 21; 22 kD; Observed band size: 22 kD)

Immunofluorescent analysis of IL-18 staining in C6 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).