Anti-IL-17A [MM17F3]

• Catalog Number: orb758853
• Category: Antibodies
• Description: Mouse monoclonal antibody to IL-17A
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: MM17F3
• Tested applications: Blocking, ELISA
• Reactivity: Mouse
• Isotype: IgG1
• Immunogen: This antibody was raised by immunising C57Bl/6 mice with IL-17A cross-linked to ovalbumin to generate a highly immunogenic complex.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IL-17A
• UniProt ID: Q62386
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Interleukin-17A; IL17A; Cytotoxic T-lymphocyte-ass
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow-cytometry using the Anti-IL-17A antibody MM17F3. Mouse splenocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of MM17F3 (orb758854, blue line) at a dilution of 1:100 for 1h at RT. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.