Anti-IL-16 [14.1]

SKU: orb669743

Description

Mouse monoclonal antibody to IL-16

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Reactivity: Human

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG
• Clone No.: 14.1
• Immunogen: The antibody was raised against IL-16 using a synthetic peptide corresponding to the predicted amino acid sequence of a hydrophilic region of IL-16, conjugated to keyhole limpet hemocyanin.
• Target: IL-16
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

LCF; interleukin 16; NPRprIL-16; IL16

Flow-cytometry using the Anti-IL-16 antibody 14.1. Human peripheral blood leukocytes were stimulated with PMA and Ionomycin in presence of Monensin for 4 hours. The cells were fixed using 2% PFA, permeabilized using 0.5% Triton and stained with anti-Fluorescein IgG antibody (4-4-20; isotype control, black line) or the rabbit IgG version of 14.1 (orb669743, pink line) at a dilution of 1:100 for 1h at RT. The blue line shows unstimulated human peripheral blood leukocytes stained with orb669743. After washing, bound antibody was detected using a goat anti-mouse IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells were analyzed using a FACSCanto flow-cytometer.

Quick Database Links

Gene Symbol

IL-16

UniProt

Q14005