Anti-IL-12/23 [ABT-874 (Briakinumab)]

• Catalog Number: orb2645662
• Category: Antibodies
• Description: Anti-IL-12/23 [ABT-874 (Briakinumab)]
• Target: IL-12/23
• Clonality: Monoclonal
• Species/Host: Human
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Concentration: 1 mg/ml
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Immunogen: This antibody was generated by phage display technology.
• UniProt ID: P29460
• Tested applications: Blocking
• Clone Number: ABT-874 (Briakinumab)
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: IL-12p40; Interleukin 12; Interleukin 23; IL12; IL
• Research Area: Immunology & Inflammation
• Note: For research use only

Flow cytometry using anti-IL-12/2 antibody ABT-874 (Briakinumab). Paraformaldehyde-fixed Jurkat cells permeabilized with 0.5% Triton were stained with the anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of ABT-874 (orb2645662, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000, and the cells were analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of Jurkat cells with anti-IL-12/23 antibody ABT-874 (Briakinumab). Immunofluorescence analysis of paraformaldehyde fixed Jurkat cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of ABT-874 (Briakinumab) (orb2645662) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645662, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.