Anti-IL-10 [9D7]

• Catalog Number: orb1089967
• Category: Antibodies
• Description: Rat monoclonal antibody to IL-10
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: 9D7
• Tested applications: ELISA, IF, IHC, IP
• Reactivity: Human
• Isotype: IgG1
• Immunogen: Rats are immunised with synthetic oligopeptide (containing an N-terminal cysteine)-carrier protein conjugates. IL-10 was conjugated to JES3. The mouse non-secreting myeloma fusion partner P3X63-AG8.653, is fused to immunised rat splenocytes to produce heterohybridomas secreting rat antibody. Bioassays were used to identify neutralising mAbs, and immunochemical screens were used to identify mAbs that bind the cytokine.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IL-10
• UniProt ID: P22301
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: Human cytokine synthesis inhibitory factor;GVHDS;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA of anti-IL-10 antibody on human rhIL-10. Binding curves of the human IgG1 chimeric version of the anti-IL-10 antibody 9D7 (blue line) and isotype control (orb256383, anti-fluorescein; orange line) to an ELISA plate coated with human rhIL-10 at a concentration of 2.5 µg/ml. A 3-fold serial dilution from 3000 ng/ml was tested in the experiment. For signal detection, a 1:4000 dilution of an anti-human IgG/HRP antibody was used.