IKK beta (Phospho-Y199) Rabbit Polyclonal Antibody

SKU: orb214092

Description

The IKK beta (Phospho-Y199) Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y199 of human IKK beta protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics, Infectious Diseases, NF-kB, Neuroscience, Signaling Pathways

Images & Validation

• Tested Applications: IHC, WB
• Dilution Range: WB: 1-500-1-1000, IHC-P: 1-100-1-200, IP: 1-10-1-100
• Reactivity: Bovine, Human, Mouse, Porcine, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding Y199 of human IKK beta protein. The exact sequence is proprietary.
• Target: IKBKB
• Purification: The antibody was purified by immunogen affinity chromatography.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

IKKB; Inhibitor of nuclear factor kappa-B kinase subunit beta; I-kappa-B-kinase beta; IKK-B; IKK-beta; IkBKB; I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta; NFKBIKB

Western blot analysis of IKK beta (Phospho-Y199) expression in HeLa LPS-treated (A), Raw264.7 LPS-treated (B), PC12 LPS-treated (C) whole cell lysates. (Predicted band size: 86 kD; Observed band size: 87 kD)

Immunohistochemical analysis of IKK beta (Phospho-Y199) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

Gene Symbol

IKBKB

UniProt

O14920, O88351, Q9QY78

NCBI Gene ID

84351, 3551, 16150