IKK alpha/beta (Phospho-S176/177) Antibody

• Catalog Number: orb304723
• Category: Antibodies
• Description: The IKK alpha/beta (Phospho-S176/177) Antibody is suitable for IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S176/177 of human IKK alpha/beta protein. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.
• Clonality: Polyclonal
• Species/Host: Rabbit
• Conjugation: Unconjugated
• Reactivity: Bovine, Gallus, Human, Monkey, Mouse, Porcine, Rat, Zebrafish
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Purification: The antibody was purified by immunogen affinity chromatography.
• Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding S176/177 of human IKK alpha/beta protein. The exact sequence is proprietary.
• UniProt ID: Q60680, O15111, O88351, Q9QY78, O14920
• Tested applications: IHC, WB
• Dilution range: WB: 1:500-1000
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: CHUK; IKKA; TCF16; Inhibitor of nuclear factor kap
• Research Area: Epigenetics, NF-kB, Neuroscience, Signaling Pathwa
• Note: For research use only
• Entrez: 3551, 16150, 1147, 84351

Western blot analysis of IKK alpha/beta (Phospho-S176/177) expression in HeLa LPS-treated (A), mouse brain (B), rat brain (C) whole cell lysates. (Predicted band size: 84; 86 kD; Observed band size: 85; 87 kD)

Immunohistochemical analysis of IKK alpha/beta (Phospho-S176/177) staining in human heart formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (Phospho-H 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.