Anti-IKB epsilon/Nfkbie Antibody

• Catalog Number: orb1184709
• Category: Antibodies
• Description: Anti-IKB epsilon/Nfkbie Antibody. Tested in ELISA, WB applications. This antibody reacts with Mouse.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Isotype: Rabbit IgG
• Immunogen: E.coli-derived mouse IKB epsilon/Nfkbie recombinant protein (Position: Q111-D364).
• Antibody Type: Primary Antibody
• Concentration: Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
• Form/Appearance: Lyophilized
• Conjugation: Unconjugated
• MW: 41 kDa
• UniProt ID: O54910
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Alternative names: Mimecan; Osteoglycin; Ogn; Og
• Note: For research use only
• Application notes: Western blot, 0.25-0.5 μg/ml/ml, Mouse ELISA, 0.1-0.5 μg/ml/ml, Mouse. Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IKB epsilon/Nfkbie using anti-IKB epsilon/Nfkbie antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions. Lane 1: mouse spleen tissue lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-IKB epsilon/Nfkbie antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for IKB epsilon/Nfkbie at approximately 41 kDa. The expected band size for IKB epsilon/Nfkbie is at 53 kDa.