Anti-IFN-gamma receptor 1 [GR20]

• Catalog Number: orb1089965
• Category: Antibodies
• Description: Rat monoclonal antibody to IFN-gamma receptor 1
• Species/Host: Rat
• Clonality: Monoclonal
• Clone Number: GR20
• Tested applications: Blocking, FC
• Reactivity: Mouse
• Isotype: IgG2a
• Immunogen: This antibody was raised by immunising rats with interferon gamma receptor.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: IFN-gamma receptor 1
• UniProt ID: P15261
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD119; Interferon gamma receptor 1; IFN gamma rece
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-IFN-gamma receptor 1 antibody GR20. Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of GR20 (orb1089966, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes with anti-IFN-gamma receptor 1 GR20. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of GR20 (orb1089966) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089966, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.