Anti-IDH3 alpha Antibody

• Catalog Number: orb382571
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IDH3A
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IH, WB
• Reactivity: Human, Mouse, Primate, Rat
• Immunogen: KLH-conjugated synthetic peptide encompassing a sequence within the center region of human IDH3 alpha. The exact sequence is proprietary.
• Antibody Type: Primary Antibody
• Dilution range: WB: 1:500:2000, IHC-P: 1:50:200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: IDH3A
• Entrez: 114096, 67834, 3419
• UniProt ID: P50213, Q99NA5, Q9D6R2
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: anti-Isocitrate dehydrogenase [NAD] subunit alpha,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of IDH3 alpha expression in SGC7901 (A), PC12 (B), CT26 (C) whole cell lysates. (Predicted band size: 39 kD; Observed band size: 40 kD)

Immunohistochemical analysis of IDH3 alpha staining in human lymph gland formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.