Anti-ICOSL [HK5.3]

SKU: orb613947

Description

Rat monoclonal antibody to ICOSL

Images & Validation

• Tested Applications: Blocking, FC
• Reactivity: Mouse

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone No.: HK5.3
• Immunogen: This antibody was raised by immunizing SD rat with mouse B7h-transfected L cells and fusing immune splenocytes with P3U1 myeloma cells and screened for binding to mouse B7h-transfected NRK cells.
• Target: ICOSL
• Purity: Purified

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Disclaimer: For research use only

Alternative Names

B7RP-1; B7-related protein 1; ICOS ligand; CD275; LICOS; B7h

Binding curve of anti-ICOSL antibody HK5.3 (orb613946) to mouse ICOSL-Fc fusion protein ELISA Plate coated with ICOSL-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 10000 to 0.1 ng/ml was performed using orb613946. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used.

Immunofluorescence staining of fixed mouse splenocytes with anti-ICOSL (inducible T-cell costimulator ligand) antibody HK5.3. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of HK5.3 (orb613946) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing weak membrane staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613946, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

ICOSL

UniProt

Q9JHJ8