Anti-HVEM [HMHV-1B18]

• Catalog Number: orb758793
• Category: Antibodies
• Description: Hamster monoclonal antibody to HVEM
• Species/Host: Hamster
• Clonality: Monoclonal
• Clone Number: HMHV-1B18
• Tested applications: Blocking, FA, FC, IP, WB
• Reactivity: Mouse
• Isotype: Hamster IgG
• Immunogen: This antibody was raised by immunising Armenian hamsters with mouse HVEM:Fc fusion protein.
• Concentration: 1 mg/ml
• Purity: Purified
• Conjugation: Unconjugated
• Target: HVEM
• UniProt ID: Q71F55
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Alternative names: CD270; TNF Receptor-like 2; ATAR; Herpes virus Ent
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry using the Anti-HVEM antibody HVEM-1B18. Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of HVEM-1B18 (orb758794, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-HVEM antibody HMHV-1B18. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of HMHV-1B18 (orb758794) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758794, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-HVEM antibody HMHV-1B18 Mouse spleen(A), mouse thymus (B) and mouse lung(C) tissue lysates (35 µg protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of HMHV-1B18 (orb758794) at 0.01 µg/ml, before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.