Anti-Human Muscle Acetylcholine Receptor [mAb 192]

SKU: orb613845

Description

Rat monoclonal antibody to Muscle Acetylcholine R

Research Area

Cell Biology, Neuroscience

Images & Validation

• Tested Applications: Blocking, ELISA, WB
• Reactivity: Human, Mouse, Rat

Key Properties

• Host: Rat
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: mAb 192
• Immunogen: Human muscle AChR.
• Target: Human Muscle Acetylcholine Receptor
• Purity: Purified
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% Proclin 300.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AChR

Immunofluorescence staining of fixed HeLa cells with anti-Human Muscle Acetylcholine Receptor antibody mAb 192. Immunofluorescence analysis of paraformaldehyde fixed HeLa cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of mAb 192 at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue).

Western Blot using anti-AChR (Human Muscle Acetylcholine Receptor) antibody mAb 192 Mouse skeletal muscle lysate (35 µg protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric mouse IgG1 version of mAb 192 at 0.03 µg/ml before detection using an anti-mouse IgG1 secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The predicted running size for AChR is 58.9 kDa.

Quick Database Links

Gene Symbol

Human Muscle Acetylcholine Receptor

UniProt

P02708