Anti-Human IL-6

• Catalog Number: orb1139222
• Category: Antibodies
• Description: Rabbit polyclonal antibody to IL6
• Clonality: Polyclonal
• Species/Host: Rabbit
• Isotype: IgG
• Conjugation: Unconjugated
• Reactivity: Human
• Form/Appearance: Liquid
• Concentration: 1ug/ul
• Buffer/Preservatives: PBS, pH 7.4 with 0.05% sodium azide.
• Purity: Purified
• Purification: Purified
• Immunogen: This antibody was prepared by repeated immunizations with recombinant human IL-6 produced in E.coli.
• Tested applications: ELISA, IHC, WB
• Dilution range: This antibody has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation and immunoblotting. It recognizes IL-6. Reactivity in other immunoassays is unknown. For immunoblotting, use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human plus 10 ng/mL E.coli LPS. For immunohistochemistry, either paraffin fix or cryofix tissue. For immunoprecipitation, preclearing with a non-specific rabbit IgG is helpful to reduce background. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as the capture antibody.
• Application notes: This antibody has been tested for use in neutralizations, ELISA, immunohistochemistry, radioimmunoassay, immunoprecipitation and immunoblotting. It recognizes IL-6. Reactivity in other immunoassays is unknown. For immunoblotting, use the supernatants or lysates of 2 x 106 endotoxin-stimulated human peripheral blood mononuclear cells (PBMC). PBMC are stimulated for 24 hours with 1% (v/v) human plus 10 ng/mL E.coli LPS. For immunohistochemistry, either paraffin fix or cryofix tissue. For immunoprecipitation, preclearing with a non-specific rabbit IgG is helpful to reduce background. For use in ELISA formats, this antibody is best used as the second antibody in combination with a monoclonal antibody as the capture antibody.
• Antibody Type: Primary Antibody
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only