TCR Valpha24-Jalpha18 Antibody

• Catalog Number: orb1993813
• Category: Antibodies
• Description: TCR alpha V24-J18 chain is a marker of invariant NK T cells (iNKT). This chain pairs mostly with TCR beta V11 chain, and together they interact with glycolipide-loaded CD1d. This interaction results in iNKT activation and cytokine production (IFN gamma, IL-4, IL-13), which takes important part in immune regulation and host response to pathogens and cancer(support of both Th1 and Th2 way), although iNKT cells represent less than 1% of total peripheral T cells.
• Target: TCR Valpha24-Jalpha18
• Clonality: Monoclonal
• Isotype: Mouse IgG1 kappa
• Conjugation: Unconjugated
• Reactivity: Human, Primate
• Concentration: 1 mg/ml
• Buffer/Preservatives: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Purification: Purified by protein-A affinity chromatography.
• Tested applications: FC, ICC, IHC-Fr
• Application notes: Flow cytometry: Recommended dilution: 1-5 µg/ml
• Clone Number: 6B11
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human TCR Valpha24-Jalpha18 (6B11) purified antibody (concentration in sample 0.56 µg/ml, GAM APC) and anti-human CD3 (UCHT1) APC antibody (20 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD3 positive TCR Valpha24-Jalpha18 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human TCR Valpha24-Jalpha18 (6B11) purified antibody (concentration in sample 0.56 µg/ml, GAM APC).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human TCR Valpha24-Jalpha18 (6B11) purified antibody (concentration in sample 0.56 µg/ml, GAM APC).